Emissive supramolecular metallacages via coordination-driven self-assembly

Credit: Pixabay/CC0 Public Domain Metallacages prepared via coordination-driven self-assembly have received extensive attention because of their three-dimensional layout and cavity-cored nature. The construction of light-emitting materials employing metallacages as a platform has also gained significant interest due to their good modularity in photophysical properties, which bring emerging applications in fields as diverse as sensing, biomedicine,…

Circular RNAs sequenced at last

Full-length circular RNAs are sequenced at high throughput using nanopores. Access options Subscribe to JournalGet full journal access for 1 year$59.00only $4.92 per issueAll prices are NET prices. VAT will be added later in the checkout.Tax calculation will be finalised during checkout.Rent or Buy articleGet time limited or full article access on ReadCube.from$8.99All prices are…

Detection of low-frequency DNA variants by targeted sequencing of the Watson and Crick strands

AbstractIdentification and quantification of low-frequency mutations remain challenging despite improvements in the baseline error rate of next-generation sequencing technologies. Here, we describe a method, termed SaferSeqS, that addresses these challenges by (1) efficiently introducing identical molecular barcodes in the Watson and Crick strands of template molecules and (2) enriching target sequences with strand-specific PCR. The…

MHC class II tetramers engineered for enhanced binding to CD4 improve detection of antigen-specific T cells

AbstractThe ability to identify T cells that recognize specific peptide antigens bound to major histocompatibility complex (MHC) molecules has enabled enumeration and molecular characterization of the lymphocytes responsible for cell-mediated immunity. Fluorophore-labeled peptide:MHC class I (p:MHCI) tetramers are well-established reagents for identifying antigen-specific CD8+ T cells by flow cytometry, but efforts to extend the approach…

Computational principles and challenges in single-cell data integration

1.Navin, N. E. The first five years of single-cell cancer genomics and beyond. Genome Res. 25, 1499–1507 (2015).CAS  PubMed  PubMed Central  Article  Google Scholar  2.Peng, G., Cui, G., Ke, J. & Jing, N. Using single-cell and spatial transcriptomes to understand stem cell lineage specification during early embryo development. Annu. Rev. Genomics Hum. Genet. 21, 163–181…

Field trial demonstrating phytoremediation of the military explosive RDX by XplA/XplB-expressing switchgrass

AbstractThe explosive hexahydro-1,3,5-trinitro-1,3,5-triazine (RDX), a major component of munitions, is used extensively on military training ranges. As a result, widespread RDX pollution in groundwater and aquifers in the United States is now well documented. RDX is toxic, but its removal from training ranges is logistically challenging, lacking cost-effective and sustainable solutions. Previously, we have shown…

Long-term wireless streaming of neural recordings for circuit discovery and adaptive stimulation in individuals with Parkinson’s disease

AbstractNeural recordings using invasive devices in humans can elucidate the circuits underlying brain disorders, but have so far been limited to short recordings from externalized brain leads in a hospital setting or from implanted sensing devices that provide only intermittent, brief streaming of time series data. Here, we report the use of an implantable two-way…

Here’s how badly millions of Americans want a new stimulus check

Last year, a Colorado restaurant owner named Stephanie Bonin started a petition on Change.org calling for recurring stimulus checks. The one-and-done coronavirus relief payments that President Trump had signed into law — one for $1,200 near the start of the pandemic, and another stimulus check for $600 at the end of 2020 — were not…

Can You Do a Full-Body Workout Two Days in a Row?

Photo: oleksboiko (Shutterstock)It’s time to bust a gym myth. I remember being taught, explicitly, as if it were a fact that my life might someday depend on, that you should never strength train the same muscle two days in a row. There is, it turns out, no such law of nature. You can train whatever…

Mantis shrimp throw deadly punches just 9 days after birth

Baby animals are often adorable, but this belies the fact that many are born with some pretty intense survival skills. This is especially true for creatures that don’t nurture their young since newborns have to fend for themselves as soon as they are brought into the world. A new study of the infamous mantis shrimp…